datura stramonium l  (Vector Laboratories)

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Clinical characteristics of patients for lectin microarray.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: Microarray

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Clinical characteristics of patients for lectin blot.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques:

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Lectin array profiling of serum samples from Hashimoto′s thyroiditis (HT) patients (n=27) and healthy control (HC) (n=26). (A) Representative results of the array profiling for HT (left) and HC (right) groups. Red boxes indicate the position of lectins with the significant difference between groups. (B) Principal component analysis (PCA) of the mean normalized signals obtained from lectin microarray. A scatter plot for principal component (PC) 1 and 2 is shown. Each dot represents the sample derived from an individual person. Different groups are indicated by color. (C) Volcano plot of log2 fold change (x axis) in lectin binding signals of HT samples vs . HC samples against the significance of change shown as the −log10 p-value (y axis). Red and green dots indicate lectins with up-regulated and down-regulated binding signals, respectively. (D) Heat map and hierarchical clustering of lectin microarray signals. Each column represents samples from an individual person. Thirteen lectins are shown on rows. (E) The binding signal of 14 lectins shows a significant difference between groups. *p <0.05, **p <0.01, ***p <0.005 and ****p <0.001. VVA, Vicia villosa lectin; MAA, Maackia amurensis lectin; STL, Solanum tuberosum lectin; Calsepa, Calystegia sepium lectin; SNA-I, Sambucus nigra I lectin; SAMB, Sambucus Sieboldiana lectin; DSA, Datura stramonium lectin; BanLec, Musa acuminata lectin; ACG, Agrocybe cylindracea lectin; PHA-E, Phaseolus vulgaris Erythroagglutinin ; MPL, Maclura pomifera lectin; PSA, Pisum sativum lectin; Gal1, Human galectin1 lectin; GS-I, Griffonia simplicifolia I lectin.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: Control, Microarray, Derivative Assay, Binding Assay

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Lectin blot verification of a small cohort of serum samples profiled by lectin array. (A) The VVA, MAA, STL, SNA-I and DSA blot using serum samples obtained from the HT (left 2-8 column) and HC group (left 9-15 column). The number above each column indicates the corresponding sample. VVA and MAA bands are shown at ~75 KDa and 100 KDa, respectively. Band for STL blot was between 75 to 100 KDa on SDS-PAGE. SNA-I and DSA bands are shown at ~60 KDa. (B) The signal intensity analysis of bands from VVA, MAA, STL, SNA-I and DSA blot. All error bars indicate SD. *p <0.05.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: SDS Page